Dr Luke Green

Clinical Medicine, School of Medicine and Population Health

Research Fellow

L.R.Green@sheffield.ac.uk
+44 114 215 9547

Room LU105, L Floor, The Medical School

Full contact details

Dr Luke Green
Clinical Medicine, School of Medicine and Population Health
Room LU105, L Floor
The Medical School
Beech Hill Road
Sheffield
S10 2RX

Profile

For enquiries, please contact – ClinMed-Operational@sheffield.ac.uk

2021-Present: The Humane Research Trust Research Fellow; University of Sheffield, UK.
2018-2021: Postdoctoral Associate; Prof. Pete Monk, University of Sheffield, UK.
2015-2018: Postdoctoral Researcher; Dr. Chris Bayliss, University of Leicester, UK.
2011-2015: Research Associate; Dr. Ingrid Scully Group, Pfizer Inc., USA.
2006-2010: PhD; Prof. Rob Read Group, University of Sheffield, UK.

Research interests

Infectious disease is a huge health burden globally and antibiotic resistance is an ever growing problem, currently attributable to ~700,000 deaths per year with projected figures rising to 10 million by the year 2050. For this reason, new therapies to replace antibiotics are crucial. Due to their rapid doubling time, bacteria have the ability to quickly evolve mechanisms to overcome drugs targeted to them, for this reason, I believe the future for anti-infectives is to target infection at the human cell level. Bacteria enter our cells by "hijacking" parts of our own cell machinery (proteins).

Our cells are encased in a membrane consisting of water-repelling fatty acids and proteins. Bacteria have to attach to and breach this membrane in order to get inside our cells. They do this by "hijacking" the proteins within the membrane. Whilst there has been extensive research into how bacteria bind our cells, no universal mechanisms (used by all bacteria) are known. Different bacterial species have been shown to hijack different proteins, which causes problems with therapeutic design. I have discovered that a family of human cell membrane proteins, the tetraspanins, are extremely important in the binding of multiple bacterial species to our cells. Targeting tetraspanins using drugs reduces bacterial binding to multiple types of human cells by greater than 50%. The tetraspanins form part of our cell membranes and act as "organisers", bringing together lots of other proteins to form large protein "islands" in the cell membrane. Bacteria do not directly attach to the tetraspanins but I hypothesise that the tetraspanins organize the proteins required for bacterial binding and it is therefore the loss of this organization which results in reduced bacterial adhesion to our cells when the tetraspanins are blocked.

My lab is interested in investigating the role of these proteins and their interactors in bacterial infection.

Publications

Show: Featured publications All publications

Featured publications

Journal articles

Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway. mBio, e0148223.
Wanford JJ, Holmes JC, Bayliss CD & Green LR (2020) Meningococcal core and accessory phasomes vary by clonal complex. Microbial Genomics, 6(5). View this article in WRRO
Green LR, Al-Rubaiawi AA, Al-Maeni MARM, Harrison OB, Blades M, Oldfield NJ, Turner DPJ, Maiden MCJ & Bayliss CD (2020) Localized Hypermutation is the Major Driver of Meningococcal Genetic Variability during Persistent Asymptomatic Carriage. mBio, 11(2).
Green LR, Dave N, Adewoye AB, Lucidarme J, Clark SA, Oldfield NJ, Turner DPJ, Borrow R & Bayliss CD (2019) Potentiation of Phase Variation in Multiple Outer-Membrane Proteins During Spread of the Hyperinvasive Neisseria meningitidis Serogroup W ST-11 Lineage. The Journal of Infectious Diseases, 220(7), 1109-1117.
Green LR, Lucidarme J, Dave N, Chan H, Clark S, Borrow R & Bayliss CD (2018) Phase Variation of NadA in Invasive Neisseria meningitidis Isolates Impacts on Coverage Estimates for 4C-MenB, a MenB Vaccine. Journal of Clinical Microbiology, 56(9).
Oldfield NJ, Green LR, Parkhill J, Bayliss CD & Turner DPJ (2018) Limited Impact of Adolescent Meningococcal ACWY Vaccination on Neisseria meningitidis Serogroup W Carriage in University Students. The Journal of Infectious Diseases, 217(4), 608-616.
Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.. Infect Immun, 79(6), 2241-2249. View this article in WRRO

All publications

Journal articles

Green LR, Issa R, Albaldi F, Urwin L, Thompson R, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway. mBio, e0148223.
Jadi PK, Dave A, Issa R, Tabbasum K, Okurowska K, Samarth A, Urwin L, Green LR, Partridge LJ, MacNeil S , Garg P et al (2023) Tetraspanin CD9-derived peptides inhibit Pseudomonas aeruginosa corneal infection and aid in wound healing of corneal epithelial cells. The Ocular Surface.
Dave N, Albiheyri RS, Wanford JJ, Green LR, Oldfield NJ, Turner DPJ, Martinez-Pomares L & Bayliss CD (2023) Variable disruption of epithelial monolayers by Neisseria meningitidis carriage isolates of the hypervirulent MenW cc11 and MenY cc23 lineages. Microbiology, 169(2), micro001305.
Parker MD, Stewart H, Shehata OM, Lindsey BB, Shah DR, Hsu S, Keeley AJ, Partridge DG, Leary S, Cope A , State A et al (2022) Altered subgenomic RNA abundance provides unique insight into SARS-CoV-2 B.1.1.7/Alpha variant infections. Communications Biology, 5. View this article in WRRO
Green LR, Cole J, Parga EFD & Shaw JG (2022) Neisseria gonorrhoeae physiology and pathogenesis. Advances in microbial physiology, 80, 35-83. View this article in WRRO
Lindsey BB, Villabona-Arenas CJ, Campbell F, Keeley AJ, Parker MD, Shah DR, Parsons H, Zhang P, Kakkar N, Gallis M , Foulkes BH et al (2022) Publisher Correction: Characterising within-hospital SARS-CoV-2 transmission events using epidemiological and viral genomic data across two pandemic waves.. Nat Commun, 13(1), 1013.
Lindsey BB, Villabona-Arenas CJ, Campbell F, Keeley AJ, Parker MD, Shah DR, Parsons H, Zhang P, Kakkar N, Gallis M , Foulkes BH et al (2022) Characterising within-hospital SARS-CoV-2 transmission events using epidemiological and viral genomic data across two pandemic waves. Nature Communications, 13(1). View this article in WRRO
Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN (2021) ACE2-independent interaction of SARS-CoV-2 spike protein with human epithelial cells is inhibited by unfractionated heparin. Cells, 10(6). View this article in WRRO
Parker MD, Lindsey BB, Leary S, Gaudieri S, Chopra A, Wyles M, Angyal A, Green LR, Parsons P, Tucker RM , Brown R et al (2021) Subgenomic RNA identification in SARS-CoV-2 genomic sequencing data. Genome Research. View this article in WRRO
Remmington A, Haywood S, Edgar J, Green LR, de Silva T & Turner CE (2021) Cryptic prophages within a Streptococcus pyogenes genotype emm4 lineage. Microbial Genomics, 7(1). View this article in WRRO
Urwin L, Okurowska K, Crowther G, Roy S, Garg P, Karunakaran E, MacNeil S, Partridge LJ, Green LR & Monk PN (2020) Corneal infection models : tools to investigate the role of biofilms in bacterial keratitis. Cells, 9(11). View this article in WRRO
Korber B, Fischer WM, Gnanakaran S, Yoon H, Theiler J, Abfalterer W, Hengartner N, Giorgi EE, Bhattacharya T, Foley B , Hastie KM et al (2020) Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus. Cell, 182(4), 812-827.e19. View this article in WRRO
The COVID-19 Genomics UK (COG-UK) consortium , Wang D, Angyal A, Brown RL, Carrilero L, de Silva TI, Green LR, Groves DC, Keeley AJ, Lindsey BB , Parker MD et al (2020) An integrated national scale SARS-CoV-2 genomic surveillance network. The Lancet Microbe, 1(3), e99-e100. View this article in WRRO
Wanford JJ, Holmes JC, Bayliss CD & Green LR (2020) Meningococcal core and accessory phasomes vary by clonal complex. Microbial Genomics, 6(5). View this article in WRRO
Green LR, Al-Rubaiawi AA, Al-Maeni MARM, Harrison OB, Blades M, Oldfield NJ, Turner DPJ, Maiden MCJ & Bayliss CD (2020) Localized Hypermutation is the Major Driver of Meningococcal Genetic Variability during Persistent Asymptomatic Carriage. mBio, 11(2).
Green LR, Dave N, Adewoye AB, Lucidarme J, Clark SA, Oldfield NJ, Turner DPJ, Borrow R & Bayliss CD (2019) Potentiation of Phase Variation in Multiple Outer-Membrane Proteins During Spread of the Hyperinvasive Neisseria meningitidis Serogroup W ST-11 Lineage. The Journal of Infectious Diseases, 220(7), 1109-1117.
Gray K, Green LR, Chaudhuri RR & Shaw JG (2019) Draft Whole-Genome Sequences of 10 Aeromonas Strains from Clinical and Environmental Sources. Microbiology Resource Announcements, 8(30). View this article in WRRO
Dave N, Green L, Lucidarme J, Oldfield N & Bayliss C (2019) Phase variation of Opa proteins in hypervirulent serogroup W meningococcal isolates. Access Microbiology, 1(1A).
Aidley J, Wanford JJ, Green LR, Sheppard SK & Bayliss CD (2018) PhasomeIt: an ‘omics’ approach to cataloguing the potential breadth of phase variation in the genus Campylobacter. Microbial Genomics, 4(11).
Green LR, Lucidarme J, Dave N, Chan H, Clark S, Borrow R & Bayliss CD (2018) Phase Variation of NadA in Invasive Neisseria meningitidis Isolates Impacts on Coverage Estimates for 4C-MenB, a MenB Vaccine. Journal of Clinical Microbiology, 56(9).
Oldfield NJ, Green LR, Parkhill J, Bayliss CD & Turner DPJ (2018) Limited Impact of Adolescent Meningococcal ACWY Vaccination on Neisseria meningitidis Serogroup W Carriage in University Students. The Journal of Infectious Diseases, 217(4), 608-616.
Oldfield NJ, Cayrou C, AlJannat MAK, Al-Rubaiawi AAA, Green LR, Dada S, Steels OD, Stirrup C, Wanford J, Atwah BAY , Bayliss CD et al (2017) Rise in Group W Meningococcal Carriage in University Students, United Kingdom. Emerging Infectious Diseases, 23(6), 1009-1011.
Scully IL, Swanson K, Green L, Jansen KU & Anderson AS (2015) Anti-infective vaccination in the 21st century—new horizons for personal and public health. Current Opinion in Microbiology, 27, 96-102.
Green LR, Monk PN, Partridge LJ, Morris P, Gorringe AR & Read RC (2011) Cooperative role for tetraspanins in adhesin-mediated attachment of bacterial species to human epithelial cells.. Infect Immun, 79(6), 2241-2249. View this article in WRRO
Holmes JC, Green LR, Oldfield NJ, Turner DPJ & Bayliss CD () Rapid Transmission of a Hyper-Virulent Meningococcal Clone Due to High Effective Contact Numbers and Super Spreaders. Frontiers in Genetics, 11.
Wanford JJ, Green LR, Aidley J & Bayliss CD () Phasome analysis of pathogenic and commensal Neisseria species expands the known repertoire of phase variable genes, and highlights common adaptive strategies. PLOS ONE, 13(5), e0196675-e0196675.
Cayrou C, Akinduko AA, Mirkes EM, Lucidarme J, Clark SA, Green LR, Cooper HJ, Morrissey J, Borrow R & Bayliss CD () Clustered intergenic region sequences as predictors of factor H Binding Protein expression patterns and for assessing Neisseria meningitidis strain coverage by meningococcal vaccines. PLOS ONE, 13(5), e0197186-e0197186.
Parker MD, Lindsey BB, Leary S, Gaudieri S, Chopra A, Wyles M, Angyal A, Green LR, Parsons P, Tucker RM , Brown R et al () periscope: sub-genomic RNA identification in SARS-CoV-2 Genomic Sequencing Data. View this article in WRRO

Chapters

Green LR, Haigh RD & Bayliss CD (2019) Determination of Repeat Number and Expression States of Phase-Variable Loci Through Next Generation Sequencing and Bioinformatic Analysis, Methods in Molecular Biology (pp. 83-92). Springer New York
Green LR, Eiden J, Hao L, Jones T, Perez J, McNeil LK, Jansen KU & Anderson AS (2016) Approach to the Discovery, Development, and Evaluation of a Novel Neisseria meningitidis Serogroup B Vaccine, Vaccine Design (pp. 445-469). Springer New York

Preprints

Green LR, Issa R, Albaldi F, Urwin L, Khalid H, Turner CE, Ciani B, Partridge LJ & Monk PN (2023) CD9 co-operation with syndecan-1 is required for a major staphylococcal adhesion pathway, Cold Spring Harbor Laboratory.
Parker MD, Lindsey BB, Shah DR, Hsu S, Keeley AJ, Partridge DG, Leary S, Cope A, State A, Johnson K , Ali N et al () Altered Subgenomic RNA Expression in SARS-CoV-2 B.1.1.7 Infections, Cold Spring Harbor Laboratory.
Partridge LJ, Urwin L, Nicklin MJH, James DC, Green LR & Monk PN () ACE2-independent interaction of SARS-CoV-2 spike protein to human epithelial cells can be inhibited by unfractionated heparin.

Grants

The Humane Research Trust Research Fellowship.
THRT PhD Scholarship.